The mixture was suspended in fresh media, serially diluted and pl

The mixture was suspended in fresh media, serially diluted and plated on soybean mannitol agar click here plates containing 10 mM MgCl2. After 18 h incubation at 30 °C, the plates were overlaid with 500 μg nalidixic acid to inhibit E. coli growth. Depending on the plasmid marker, either apramycin (1 mg) or thiostrepton (0.5 mg) were overlaid to select for exconjugants. Exconjugants usually appeared on the 5th day after drug overlay. Individual

exconjugants were taken forward for further generations. Proteins separated by SDS-PAGE were transferred to nitrocellulose membrane by semi-dry electroblotting. After electrophoresis, the gel was soaked in ice-chilled transfer buffer for 20 min. On the anode plate of the apparatus, three layers of Whatman-3 filter papers were placed.

Nitrocellulose membrane soaked in Towbin transfer buffer was placed on the filter paper wad. Then the gel was placed on the membrane, followed by a wad of four Whatman-3 papers soaked with transfer buffer. The cathode plate and safety cover CB-839 purchase were placed in position and blotting was done at 250 mA for 2 h. rDnrO protein 50 μg was incubated with 20 ng of DNR for 30 min at room temperature and was centrifuged in a 10-kDa membrane centrifugal concentrator (as shown by Prasad et al., 2003) for 20 min at 6000 g at 4 °C. The retentate was suspended in 10 mM Tris pH 7.5, and analyzed using a spectrophotometer at 590 nm for the presence of DNR complexed with DnrO. Streptomyces lividans TK24 carrying

pIJ8660/dnrNO plasmid was grown in nitrate-defined yeast extract medium for 36 h at 30 °C. It was further subcultured in nitrate-defined yeast extract medium with 2 ng mL−1 DNR and incubated for 48 h at 30 °C. Mycelium was quickly washed with phosphate-buffered saline (PBS) and the expression of EGFP was visualized using a confocal microscope (Leica) at 60 × magnification. The 37-bp DnrO-binding sequence was used to construct a 3D model using ‘model it server’ ( ADAMTS5 The structure of DNR was downloaded from protein data bank ( as a pdb file. autodock ( was used to dock the DNR molecule to the 37-bp DNA to find preferred binding sequences. DNA-binding ELISA (Renard et al., 2001) was performed using Sigma screen streptavidin-coated high-capacity microplates. Biotinylated 511-bp (intergenic region of dnrN and dnrO genes carrying the 37-bp DnrO-binding site) DNA (10 ng) in TE was immobilized to ELISA plates at room temperature. Wells were washed thoroughly with PBS containing 0.1% Tween-20. Different amounts of rDnrO were added to test the binding capacity to immobilized DNA and were incubated for 1 h at 30 °C. Unbound DnrO was washed with PBS containing 1% Tween-20, following which, primary (anti-DnrO) antibody was added.

For instance, in many HIV-infected cohorts, cigarette smoking, re

For instance, in many HIV-infected cohorts, cigarette smoking, recreational drug use (including cocaine use), increased alcohol intake and reduced physical activity are highly prevalent [11]. These factors may also affect the risk of neurocognitive disorders (HIV-associated neurocognitive disease and dementia), non-AIDS-associated

malignancies, liver disease, diabetes, and renal and osteoporotic bone diseases. Some Tyrosine Kinase Inhibitor Library cost cohort studies have already suggested that modification of risk factors can decrease the incidence of non-AIDS-defining chronic conditions, including CVD [6]. Hence, it is important to screen and manage risk factors for long-term age-related diseases that increasingly affect the HIV-infected population. Most studies that have examined the contribution of HIV infection to mortality, including those discussed above, do not have an ideal control population. Hence, considerable caution needs to be exercised when attributing relative risk of mortality caused by HIV itself as opposed to unattributed associated confounding variables, particularly lifestyle factors. Even a supposedly ideal control population, such as individuals at high risk of HIV infection but who remain uninfected, might differ in terms of host

factors that govern both infectability and mortality. A study from Denmark that carefully matched cases and controls concluded that mortality in patients without risk factors on successful Protein Tyrosine Kinase inhibitor HAART therapy is almost identical to that of the non-HIV-infected population [12]. It is important to further define the relationship between HIV infection and mortality, especially those factors that can be modified to attenuate any risk. Screening tools and risk calculators for the general population have been developed for some common noncommunicable chronic diseases, as best exemplified

by coronary heart disease (CHD), fragility fractures, diabetes and renal disease. Personalized risk prediction aims to estimate, communicate and monitor risk to motivate adherence to lifestyle change or therapies, and to allocate scarce prevention dipyridamole resources and strategies appropriately. The World Health Organization (WHO) has recently focused on noncommunicable diseases (NCDs), as they are the leading cause of death globally, killing more people each year than all other causes combined [13]. The WHO has recognized that, contrary to popular opinion, available data demonstrate that nearly 80% of NCD deaths occur in low- and middle-income countries [13]. CVD is one of the leading causes of death in the UK and is largely preventable [14]. In 2008, there were more than 191 000 deaths attributable to heart and circulatory disease in the UK, including 88 000 deaths from CHD and a further 43 000 from stroke.

“We aimed in this study to

identify the significan

“We aimed in this study to

identify the significant latent pathways and precise molecular mechanisms underlying the syndrome of vasculitis. Agilent dual-channel data of peripheral selleck kinase inhibitor blood mononuclear cells (PBMCs) from healthy controls and vasculitis patients were downloaded from EBI Array Express database. Differentially expressed genes (DEGs) between normal and vasculitis PBMCs samples were selected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out to identify significant biological processes and pathways. DEGs were matched to NetBox software database to obtain LINKER genes with statistical significance. Protein–protein interaction (PPI) network was constructed with LINKER genes and DEGs according to STRING database. Latent pathway identification analysis (LPIA) buy IWR-1 was used to identify the most significant interactions among different pathways involved by DEGs. A total of 266 DEGs were selected. GO and KEGG pathway analysis showed that the up-regulated genes were significantly enriched in

defense and wounding response; the down-regulated genes were enriched in immune response. The modules analysis of PPI network suggested that ISG15 and IFIT3 were the potential biomarkers for vasculitis. The results of LPIA showed that NOD-like receptor signaling pathway and shigellosis related pathway were the two most significant latent pathway interactions for vasculitis. ISG15 and IFIT3 were the potential biomarkers for vasculitis identification. NOD-like receptor signaling Ketotifen pathway and shigellosis related pathway were the most significant latent pathway interactions for vasculitis. Moreover, LPIA was a useful method for revealing systemic biological pathways and cellular mechanisms of diseases. “
“T cell abnormalities with a focus on Th17 cells have been associated with the pathogenesis of systemic sclerosis (SSc) and interstitial lung disease (ILD). The aim of this study was to evaluate serum levels of interleukin (IL)-17,

IL-21 and IL-23 in SSc patients and to assess their relationship with ILD-SSc. Thirty-eight patients with SSc and 39 healthy controls were recruited. Serum IL-17, IL-21 and IL-23 levels were examined using enzyme-linked immunosorbent assay (ELISA). Lung involvement of SSc patients was assessed functionally (diffusing capacity of the lung for carbon monoxide [DLCO], body plethysmography) and radiologically (using average disease extent on high resolution computed tomography [HRCT] of the lungs according to the percentage of interstitial changes and quantified with a 30-point Warrick score) in 29 SSc patients. Serum IL-17 and IL-23 levels were significantly decreased and IL-21 levels were elevated in SSc patients when compared with controls (P < 0.001, P < 0.001, P < 0.01, respectively).

Cidofovir was shown in a large multicentre study to provide no ad

Cidofovir was shown in a large multicentre study to provide no additional

benefit to HAART alone [105] and these results have been confirmed in retrospective analyses of pooled data from prior cohort or observational studies [106,107]. Similarly, cytarabine, either intravenously or intrathecally, failed to demonstrate additional benefit to ARV treatment, albeit this study was conducted pre-HAART [108]. Hence, HAART remains the only therapeutic option. The choice of HAART should consider probable CNS penetration as one study has shown a better outcome with drugs based on their CNS penetration score [110]. There is no therapy that has been identified MAPK inhibitor as effective in preventing PML. From a predicted survival of 10% at one year, 50% of patients receiving HAART now survive for this length of time [110] and some patients enter true remission of disease with stabilization of neurological morbidity and the development of atrophy and gliosis on MRI. Also, since the impact of HAART on PML may be less than for other

focal neurological lesions, the relative contribution of PML to the incidence of focal lesions in the brain may have increased [100]. Cytomegalovirus (CMV) is a member of the human β-herpesviruses. Like other members, it has the ability to establish lifelong persistent and latent infection after primary exposure. Lenvatinib In the context of immunodeficiency, particularly cell-mediated, this may result in severe primary or reactivated clinical disease. Nearly all men who have sex with men (MSM) are seropositive whereas in heterosexuals and injection drug users, the rate is 50–75% [111]. With clinical progression of HIV, latent CMV reactivates, leading to viraemia and, in a proportion, end-organ disease. Prior to the advent of HAART, observational studies demonstrated that 20–40% of patients with AIDS developed CMV disease, with many more patients having

Erastin manufacturer evidence of disease at post mortem. End-organ disease incidence becomes substantially higher when the CD4 count falls to <50 cells/μL. The major sites of CMV disease are the retina, which accounts for approximately three-quarters of cases, the GI tract, the lung, the liver and biliary tract, the heart, adrenal glands and the nervous system (encephalitis and polyradiculitis). The widespread uptake of HAART has radically altered the epidemiology with most patients starting treatment before they become at risk for CMV disease. Nervous system infection accounts for <1% of clinical CMV disease [112,113]. Clinical signs and symptoms are insensitive and difficult to distinguish from AIDS-dementia complex.

”47 These include not only the African meningitis belt countries

”47 These include not only the African meningitis belt countries Luminespib clinical trial (the guidelines note that the dry season varies from country to country and extends the time frame to 9 months—from October to June) but also those countries in sub-Saharan Africa outside the traditional meningitis belt where recent epidemics have occurred, including the Congo and Tanzania.47 The guidelines also recommend vaccination for the usual groups of travelers who may have prolonged close contact with the local population

in these areas, but specify this may include medical personnel and those using public transportation. In addition to areas with active epidemics, vaccination may also be warranted for travelers to areas with “heightened disease activity,” including industrialized nations where sporadic cases of disease have been reported in the previous 6 months. In developed countries, S1P Receptor inhibitor travelers should

follow the recommendations of the destination country.47 Although vaccination against serogroup C with a monovalent vaccine is required for all Canadian children, CATMAT notes that this routine vaccination does not provide sufficient protection to individuals traveling to destinations where disease due to other serogroups is reported. Broad serogroup protection is warranted due to this risk, and the preferred vaccine is a glycoconjugate quadrivalent meningococcal vaccine due to its “significant advantages over polysaccharide vaccines including better immune memory, longer duration of efficacy, lack of hyporesponsiveness Non-specific serine/threonine protein kinase with booster doses, and possible reduction of bacterial carriage rates.”47 For the vast majority of travelers, ie, those not making pilgrimages to Saudi Arabia or those not entering college where vaccination is required (chiefly in the United States), the decision to vaccinate is based essentially on an assessment of the risk to the individual of developing

disease and/or of becoming a carrier of infection. This assessment must account for destination, nature and duration of potential exposure, age, and overall background health of the traveler (ie, host factors) (Figure 4). Because meningococcal vaccines are associated with relatively few adverse events and contraindications, these aspects hardly ever need to be considered. Obviously, vaccination should be recommended for all travelers visiting destinations with outbreaks or epidemic situations, wherever that might be, except those who have been vaccinated within the past 3 years. There are Web sites that can advise clinicians on active areas, such as, developed by a WHO/PATH partnership. As noted above, most expert groups recommend vaccination against meningococcal disease for at least some travelers with destinations in the African meningitis belt.

It is known that potato tubers are highly susceptible to D dadan

It is known that potato tubers are highly susceptible to D. dadantii 3937 colonization. Mutants in genes well known for their contribution to pathogenicity, such as pectate lyase and hrp, retain the wild-type learn more virulence in this tissue, and only mutant strains with severe defects in virulence show differences when compared with the wild type (Lopez-Solanilla et al., 2001). It should be pointed out that the two plant tissues are very

different. In chicory, the assays were conducted on leaves whereas in potato the assay was conducted on a storage organ tissue. It could be expected that the plant defence response to Dickeya would be stronger in the leaves than in tubers, as the latter contains mainly starch. The Tat system may be important to export factors find more involved in counteracting these plant responses. Also, it has to be noted that roles of motility and chemotaxis have been demonstrated recently in D. dadantii pathogenesis (Antunez-Lamas et al., 2009); therefore, the decrease in the virulence of the D. dadantii tat mutant on chicory leaves might be related to the observed impairment in motility. One of the potential Tat-dependent proteins involved in D. dadantii 3937 virulence is

PehX (ABF00-14958, Table 1), described as a polygalacturonase located in the periplasm and culture supernatant (Kazemi-Pour et al., 2004). We compared the polygalacturonase activity of Mtat and wild-type strains on KB plates containing polygalacturonic acid (2%), and no significant differences in clearing zone diameters surrounding inoculation points were observed after a 24-h incubation at 28 °C (data not shown). Taking into account that D. dadantii has three additional

polygalacturonases (PehN, PehV and PehW) (Nasser et al., 1999; Hugouvieux-Cotte-Pattat et al., 2002), all predicted as Tat-independent proteins, we assume that the total polygalacturonase activity corresponding to four polygalacturonases in the wild type was similar, at least in plate assays, to that of the Mtat strain. An analysis of the virulence and growth of a ΔpehV–pehW–pehX triple mutant in D. dadantii showed a reduction of 30% of the rotting region new on chicory leaves and a weak reduction (13%) of macerated tissues in potato tubers. Also, no growth defects were observed when the same triple mutant was grown with polygalacturonate as the sole carbon source (Nasser et al., 1999). These results are in agreement with our finding that the tat mutant, where PehX would be mislocated, showed diminished virulence in chicory leaves, but similar virulence in potato tubers and similar behaviour in polygalacturonate plates as regarding the wild-type strain. Also, it is known that the different pectolytic enzymes produced by D. dadantii are not equivalent, because virulence requires only some of them in specific plant hosts (Boccara et al., 1988).

Mount The KCC2-full-length (FL), KCC2-ΔNTD and KCC2-C568A fragme

Mount. The KCC2-full-length (FL), KCC2-ΔNTD and KCC2-C568A fragments were subcloned into pBluescript AZD2281 research buy SK− (Stratagene, La Jolla, CA, USA) and sequenced. The fragments were then

excised and subcloned into the hnestin 1852/tk promoter vector for pronuclear injection and a pcDNA3 vector for cell culture experiments. The expression cassettes were excised from the vector backbone, purified, and used for pronuclear injection of fertilized mouse (B6D2F1) oocytes. Pronuclear injection and implantation of oocytes into pseudopregnant mice was performed by Karolinska Center for Transgene Technologies. Pregnant dams with embryos at embryonic days (E)9.5–18.5 were killed by spinal dislocation, and the embryos were rapidly dissected out. Pups were collected at birth [postnatal day (P)0]. The transgenic embryos and pups were identified by PCR, using yolk sac or tail DNA as a template. A sense primer complementary to hnestin was combined with an antisense primer complementary to the KCC2 sequence. KCC2 expression assayed by immunohistochemistry (see below) verified an overexpressed protein. Animals were treated according to European Communities Council guidelines (directive 86/609/EEC). Embryos were fixed for 4 h or overnight in 4% paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4, and thereafter cryoprotected A-769662 overnight in 30% sucrose in PBS. The

embryos were then embedded in mounting medium (Tissue-Tek) and rapidly frozen, and 12-μm sections were serially collected in a cryostat (Leica CM3050 S; Leica Microsystems Nussloch GmbH, Germany). Sections were rinsed in PBS and blocked and permeabilized in 5% donkey serum (Jackson Immunoresearch Laboratories, West Grove, PA, USA), 1% bovine serum albumin (Sigma-Aldrich, St Louis,

MO, USA) and 0.3% Triton X-100 (Sigma-Aldrich) in PBS for 45 min, followed by overnight incubation with the primary antibody in a moist chamber. See Table 1, for a full list of the primary antibodies used. The 4.1N antibody Rutecarpine was a kind gift from Dr Kari Keinänen (Li et al., 2007). The following day, the sections were washed in PBS and then incubated for 1.5 h with secondary Cy3- or FITC-conjugated antibodies (Jackson Immunoresearch Laboratories) at a 1 : 400 dilution. When the distribution of actin microfibers was investigated, 50 μg/mL FITC- or TRITC-conjugated phalloidin (Sigma-Aldrich) was added to the solution. After subsequent PBS washes, the sections were mounted in Vectashield Hard Set mounting medium (Vector Laboratories, Burlingame, CA, USA). Primary antibodies were titrated to determine the optimal dilutions, and control slides were included with the respective primary antibody omitted. The sections were analyzed in a fluorescent (Zeiss AxioExaminer D1; 10 × and 40 × objectives) or confocal (Leica TCS-SP; 40 × objective) microscope. The mouse neural stem cell line C17.

Chemoprophylaxis was discontinued for side effects in 19 (13%) ch

Chemoprophylaxis was discontinued for side effects in 19 (13%) children. The reported side effects for atovaquone-proguanil, mefloquine, doxycycline, and chloroquine (with or without proguanil) were 13 (19%), 3 (5%), 2 (13%), and 1 (20%), respectively (p = 0.09). Compliance rates relating to atovaquone-proguanil and mefloquine, the most frequently used prophylaxis, were similar (73%

vs 67%, p = 0.56). Compliance selleck kinase inhibitor significantly varied with destination, whatever the drug (South America 29%, Indian Ocean 44%, Asia 62%, and Africa 80%, p < 0.0005). Independent variables significantly associated with low compliance relating to atovaquone-proguanil or mefloquine (Table 3) were age <5 years, destination (Indian Ocean and Asia), and monoparental family. Compliance was identical between VFR and tourist children, irrespective of the duration of the trip or the type of chemoprophylaxis. Parents reported full compliance with

all the measures to minimize food- and water-related diseases for only 51 (31%) children. Eighty percent of the children did not drink tap water, but other recommendations regarding food preparation and consumption were less frequently respected. Families were significantly more compliant Sunitinib cost with all recommended measures if the child was under 2 years in univariate analysis (OR = 4.38 [2.15–8.94]). VFR status, maternal age, familial features, health or travel insurance status, and duration of stay were not associated with greater compliance after adjustment (data not shown). This prospective study is the first in France to evaluate compliance of children traveling overseas after counseling at the travel medicine center. The principal outcome of the study is that compliance ≥80% was achieved for routine vaccine updates, yellow fever immunization, the use of repellents, and drinking bottled water, solely. Other measures were less frequently followed. As shown, an appointment at a travel

medicine center is an opportunity to update routine vaccinations. The overall 71% compliance with vaccines may be related to the fact that the yellow fever vaccine (compliance 100%) is sometimes mandatory and also only available in travel medicine centers in France. As some parents visited the mTOR inhibitor center for this vaccination, they might have accepted the other immunizations more easily. Compliance with hepatitis A and typhoid vaccines was also close to 75%, higher than compliance reported in another study recently conducted in adults traveling overseas.[11] The 66% malaria chemoprophylaxis compliance is consistent with other studies.[12-14] Reasons previously reported for poor compliance are destination[15, 16] and young age[14, 17, 18] (as in our patients), as well as purpose of the trip (VFR or tourism) and malaria prophylaxis tolerance[19] (neither significant in this study). In fact, VFR people are an extremely varied group.

This finding may help clinicians in treatment decisions “

This finding may help clinicians in treatment decisions. “
“Oral health inequalities are the measures by which equity in oral health is tracked. Despite widespread improvement in children’s dental health globally, substantial socio-economic disparities persist and may be worsening. Quantify 10-year changes in child caries occurrence by socio-economic position in a Southern Brazilian city and compare oral health inequalities over time. Representative surveys of dental caries in children (age <6 years) in Canoas, Brazil, were conducted in 2000 and 2010 following standardized methods. For each survey year, we calculated disparities by socio-economic MI-503 solubility dmso position

(maternal education and family income) in age- and sex-standardized caries occurrence (prevalence: dmft > 0; severity: mean dmft) using absolute measures (difference and Slope Index of Inequality) and relative measures (ratio and Relative Index of Inequality). Comparing 2010 to 2000, caries occurrence was lower in

all socio-economic strata. However, reductions were more pronounced among socio-economically advantaged groups, yielding no improvement in children’s oral health disparities. Some disparity indicators were consistent with increasing inequality. Overall, dental caries levels among children in Canoas improved, but inequalities in disease distribution endured. Concerted public health efforts targeting socio-economically disadvantaged groups are needed to achieve greater equity in children’s oral health. “
“To investigate risk factors for the occurrence of traumatic dental injuries (TDI) at 4 years of age. Prospective cohort Apoptosis inhibitor study. A birth cohort (n = 500) was recruited from the public healthcare system in São Leopoldo, Brazil. Demographic, socioeconomic, anthropometric, and behavioral variables were collected at 6 months, 1 year, and 4 years of age. Clinical examinations at 4 years of age were carried out by a single examiner using the Andreasen classification. Poisson

regression was used to determine risk factors for the occurrence of TDI at 4 years of age. A total of 23.7% of the children (80/337) exhibited TDI at 4 years of age. The risk of TDI was 35% lower among children who had been breastfeed for ≥6 months relative Dimethyl sulfoxide risk (RR 0.65; 95% CI 0.43-0.97) and more than twofold higher among those who were bottle fed ≥ three times a day (RR 2.37; 95% CI 1.10–5.11) at 12 months of age. Higher household income in the first year of life and greater height at 4 years of age were significantly associated with the outcome. The identification of behavioral, socioeconomic, and anthropometric risk factors for TDI in early childhood can contribute to the elaboration of prevention strategies. “
“The aetiology of isolated clefts of the lip and/or palate remains obscure. Unaffected family members are treated as if their genetic risks are equivalent and low.

Reward, but not movement, correlates were impacted by changes in

Reward, but not movement, correlates were impacted by changes in context, and neither correlate type was affected by reward manipulations (e.g. changing the expected location of a reward). This suggests that the PPTg conjunctively codes both reward and behavioral information, and that the reward information is processed in a context-dependent manner. The distinct anatomical distribution of reward and movement cells emphasizes different models of synaptic control by PPTg of DA burst firing in the VTA and SN. Relevant to both VTA and SN learning systems, however, PPTg appears to serve as

a sensory gating mechanism to facilitate reinforcement learning, while at the same time provides reinforcement-based guidance of ongoing goal-directed behaviors. “
“Marijuana has been used to relieve pain for centuries. The analgesic

mechanism of its constituents, the cannabinoids, was only revealed after the discovery of cannabinoid receptors (CB1 and CB2) two decades ago. The subsequent identification of the endocannabinoids, anandamide and 2-arachidonoylglycerol (2-AG), and their biosynthetic and degradation enzymes discloses the therapeutic potential of compounds targeting the endocannabinoid system for pain control. Inhibitors of the anandamide and 2-AG degradation enzymes, fatty acid amide hydrolase and monoacylglycerol lipase, respectively, may be superior to direct cannabinoid receptor ligands as endocannabinoids are synthesized on demand and rapidly degraded, focusing action at generating sites. Recently, IDH inhibitor clinical trial a promising strategy for pain relief was revealed in the periaqueductal gray (PAG). It is initiated by Gq-protein-coupled receptor (GqPCR) activation of the phospholipase C–diacylglycerol lipase enzymatic cascade, generating 2-AG that produces inhibition of GABAergic transmission (disinhibition) in the PAG, thereby leading to analgesia. Here, we introduce the antinociceptive properties of exogenous cannabinoids and endocannabinoids, involving their

biosynthesis and degradation processes, particularly Protirelin in the PAG. We also review recent studies disclosing the GqPCR–phospholipase C–diacylglycerol lipase–2-AG retrograde disinhibition mechanism in the PAG, induced by activating several GqPCRs, including metabotropic glutamatergic (type 5 metabotropic glutamate receptor), muscarinic acetylcholine (M1/M3), and orexin 1 receptors. Disinhibition mediated by type 5 metabotropic glutamate receptor can be initiated by glutamate transporter inhibitors or indirectly by substance P, neurotensin, cholecystokinin and capsaicin. Finally, the putative role of 2-AG generated after activating the above neurotransmitter receptors in stress-induced analgesia is discussed. “
“The locus coeruleus (LC) regulates sleep/wakefulness and is densely innervated by orexinergic neurons in the lateral hypothalamus. Here we used small interfering RNAs (siRNAs) to test the role of LC orexin type 1 receptor (OxR1) in sleep–wake control.