CONCLUSION: XSLJZD could restore the gastric emptying rate and im

CONCLUSION: XSLJZD could restore the gastric emptying rate and improve symptoms. However, the evidence remains weak due to the poor methodological quality of the included studies. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.”
“Background: The purpose of this study is to evaluate expression of metabolism-related proteins in primary unknown metastatic carcinoma (PUMC) and associated implications for treatment. Methods:

A tissue microarray containing 77 cases of PUMC was constructed and immunohistochemical staining was used to evaluate expression of the following proteins: Glycolysis-related: Glut-1, find more carbonic anhydrase (CA) IX, and monocarboxylate transporter (MCT) 4; Glutaminolysis-related: glutaminase1 (GLS1), glutamate dehydrogenase (GDH), and amino acid transporter-2 (ASCT2); and Mitochondrial-related: ATP synthase, succinate dehydrogenase (SDH) A, and SDHB. The association between immunohistochemical staining results and clinicopathologic parameters was evaluated. Results: The expression of metabolism-related proteins was different depending on the histologic subtype. Compared to other subtypes, squamous cell carcinomas (SQ) expressed more Glut-1 (p = 0.028), while adenocarcinomas (AD) expressed more SDHB in the stroma (p = 0.025). The expression of metabolism-related proteins was also different

depending on the clinical subtypes. Glut-1 was expressed most in the nodal type and the least in carcinomatosis type, when compared to other subtypes (p = 0.021). The metabolic P505-15 phenotypes also showed other

trends: when the stroma showed no glutaminolysis, the tumor mostly invaded lymph node, bone, and brain, while the tumor invaded regions other than lymph node, bone, and brain when the stroma showed glutaminolysis (p = 0.003). When the selleckchem stroma showed the mitochondrial metabolic type, the histologic subtype was mainly AD, but the non-mitochondrial type was associated more with SQ (P = 0.049). Conclusion: For PUMC, the expression of metabolism-related proteins, such as Glut-1 and SDHB, differs in the tumor or stroma depending on the clinical and histologic tumor subtype.”
“Given the unprecedented size of three-dimensional ultraspectral sounder data with high spectral resolution, lossless compression is preferable to avoid substantial degradation of the geophysical retrieval. A lossless compression method for ultraspectral sounder data is therefore developed. A quantized-principal-component-analysis-based scheme is presented by combining 3D prediction, positive mapping, and histogram packing using binary indexing vectors (positive packing) followed by a range coder. In order to achieve the optimal trade-off between residual errors and side information, an algorithm is proposed to determine adaptively the number of selected PCs and quantization parameters. Numerical experiments show that the proposed method outperforms the state-of-the-art methods (i.e.

its relation to autoimmune disease has not been elucidated Here,

its relation to autoimmune disease has not been elucidated. Here, we focused on interleukin (IL)-17, which is closely related to the pathogenesis of multiple sclerosis, and investigated the effect of ET receptor blockers on the production of IL-17 by T lymphocytes. Main methods: Lymph node cells from mice at 8 days post-immunization with MOG(35-55) were stimulated in vitro with MOG(35-55) in the presence or absence of an ET receptor blocker (BQ123 for ETA or BQ788 for ETB). Naive T cells from mice were subjected to an in vitro model of Th17 differentiation, and ET-mediated IL-17 production

was investigated under the states of Th17 differentiation and activation. Key findings: Fosbretabulin concentration ELISA revealed that MOG(35-55)-induced IL-17 production was significantly inhibited by BQ123 but check details not BQ788. Consistent with the ELISA results for IL-17. the frequency of CD4(+) T cells producing IL-17 but not IFN-gamma was reduced by BQ123. Under the differentiating state from naive T cells to Th17 cells, the spontaneous release of IL-17 from CD4(+) T cells was increased, which was insensitive to BQ123, indicating that ET/ETA signaling

did not affect Th17 differentiation. After the time period of Th17 differentiation, however, the increase in IL-17 production by restimulation of the cells with anti-CD3 plus anti-CD28 antibodies was significantly inhibited by BQ123. Significance: We demonstrated that ET/ETA signaling plays a crucial role in IL-17 production by Th17. BQ123 might be expected to be a future therapeutic drug for multiple sclerosis. (C) 2014 The Authors. Published by Elsevier Inc.”
“Matrix metalloproteinases (MMPs) are enzymes thought to be involved in tumor invasion. We selleck screening library hypothesized that MMP-2 and MHP-11 overexpression was associated with the aggressiveness of ovarian carcinoma. This study was performed on samples from 100 patients with stage III ovarian carcinomas treated surgically between 1990 and 2000. Immunohistochemical staining was performed on ovarian tumors and peritoneal implants using

monoclonal antibodies. Overexpression was defined as more than 10% of cells expressing the marker Multivariate analyses showed that only MMP-2 overexpression by cancer cells in peritoneal implants was associated with a significant risk of death by disease (hazard ratio, 2.65; 95% confidence interval, 1.41-4.97; P =.003). MMP-11 overexpression was not predictive of survival. These results suggest that MMP-2 overexpression by cancer cells in peritoneal implants and not in the primary ovarian cancer is predictive of ovarian cancer prognosis and more likely reflects the presence of particularly aggressive clones of cancer cells.”
“Background: The aim of this study was to evaluate efficacy and tolerability of levetiracetam (LEV) in patients with different epilepsy syndromes. Methods: We evaluated epileptic patients seen in the previous 18 months, including all patients with present or past exposure to LEV.

3; 95% confidence interval, 1 1-4 7) However, door-to-ECG time a

3; 95% confidence interval, 1.1-4.7). However, door-to-ECG time and door-to-balloon time were not factors related to hospital mortality. Conclusion: STEM patients presenting without chest pain showed higher baseline risk and hospital mortality than patients presenting with chest pain. ECG acquisition and primary PCI was delayed for patients presenting with non-chest

pains, but not influencing hospital mortality. Efforts to reduce pre-hospital time delay for these patients are necessary.”
“In this study, we investigate two factors that can hinder the performance of constant-pH molecular dynamics methods in predicting protein pK(a) values, using hen egg white lysozyme as a test system. The first factor is related to the molecular definition and pK(a) value of model compounds in the Poisson-Boltzmann framework. We address this by defining the model compound as a molecular fragment with an associated selleck kinase inhibitor pK(a) value that is calibrated against experimental data, which results in a decrease Selleckchem GSK621 of 0.12 units in pK(a) errors. The second addressed factor is the possibility that detrimental structural distortions are being introduced in the simulations by the underlying molecular mechanics force field. This issue is investigated by analyzing how the gradual structural rearrangements affect the predicted pK(a) values. The two GROMOS force fields studied here (43A1 and 53A6) yield good

pK(a) predictions, although a time-dependent performance is observed: 43A1 performs better after a few nanoseconds of structural reorganization (pK(a) errors of similar to 0.45), while NU7026 53A6 gives the best prediction right at the first nanosecond (pK(a) errors of 0.42). These results suggest that the good performance of constant-pH molecular dynamics methods could be further improved if these force field limitations were overcome. Proteins 2011; 79:3437-3447. (C) 2011 Wiley-Liss, Inc.”
“The present study was performed in the cities of Faisalabad and Sargodha, and pediatric in-patient

prescriptions were collected from four private and D.H.Q hospitals of these two cities. The collected data of 1420 prescriptions was analyzed for drug interactions by using the software developed by the Medical Letter. The drug interactions found were divided into severe, moderate and mild depending on the type of effect produced. The results showed that the public sector showed 820 drug interactions, that is 74.55%; on the other hand, the private sector showed 130 drug interactions, that is 40.63%. The private sector hospital of Faisalabad showed only 40 drug interactions, that is 20%; while 90 drug interactions, that is 75% were found in private sector hospital of Sargodha. The public sector hospital of Sargodha showed 390 drug interactions, 92.86% and 430 drug interactions, that is 63.24% were found in public sector hospital of Faisalabad.

We attempted to create a predictive model of complications in pat

We attempted to create a predictive model of complications in patients undergoing benign intracranial tumor resection. METHODS:

We performed a retrospective cohort study involving patients who underwent craniotomies for benign intracranial tumor resection during the period 2005-2011 and were registered in the National (Nationwide) Inpatient Sample database. A model for outcome prediction based on individual patient characteristics was developed. RESULTS: There were 19,894 patients who underwent benign tumor resection. The respective inpatient postoperative incidences were 1.3% for death, 22.7% for unfavorable discharge, 4.2% for treated hydrocephalus, 1.1% for P5091 molecular weight cardiac complications, 0.9% for respiratory complications, 0.5% for wound infection, 0.5% for deep venous thrombosis, 2.3% for pulmonary embolus, and 1.5% for acute renal failure. Multivariable analysis identified risk factors independently associated with the above-mentioned outcomes. A model for outcome prediction based on patient and hospital characteristics was developed and subsequently validated in a bootstrap sample. The models demonstrated good CH5424802 discrimination with areas under the curve of 0.85, 0.76, 0.72, 0.74, 0.72, 0.74, 0.76, 0.68, and 0.86 for postoperative risk of death, unfavorable discharge, hydrocephalus, cardiac complications, respiratory

complications, wound infection, deep venous thrombosis, pulmonary embolus, and acute renal failure. GSK2126458 The models also had good calibration, as assessed by the Hosmer-Lemeshow test. CONCLUSIONS: Our models can provide individualized estimates of the risks of postoperative complications based on preoperative conditions

and potentially can be used as an adjunct for decision making in benign intracranial tumor surgery.”
“Sponges are among the earliest diverging lineage within the metazoan phyla. Although their adult morphology is distinctive, at several stages of development, they possess characteristics found in more complex animals. The T-box family of transcription factors is an evolutionarily ancient gene family known to be involved in the development of structures derived from all germ layers in the bilaterian animals. There is an incomplete understanding of the role that T-box transcription factors play in normal sponge development or whether developmental pathways using the T-box family share similarities between parazoan and eumetazoan animals. To address these questions, we present data that identify several important T-box genes in marine and freshwater sponges, place these genes in a phylogenetic context, and reveal patterns in how these genes are expressed in developing sponges. Phylogenetic analyses demonstrate that sponges have members of at least two of the five T-box subfamilies (Brachyury and Tbx2/3/4/5) and that the T-box genes expanded and diverged in the poriferan lineage.

The resultant RFP generated 23 letters of intent from industry an

The resultant RFP generated 23 letters of intent from industry and academic institutions, of which three were chosen for funding.\n\nAssessing clinical needs is a necessary first step in developing new technologies. A multi-faceted approach assures that the views of interested stakeholders AZD9291 concentration are represented and can influence success.”
“Petrography, isocon analysis and singular value decomposition analysis of jadeitites and metasomatic rocks surrounding them from the Nishisonogi metamorphic rocks revealed that the metasomatic rocks developed outside the stability field of jadeite +

quartz after jadeitites had been included by a serpentinite melange in a subduction channel possibly during exhumation. The jadeitites occur at two localities, Tone

and Mie in Nagasaki City, from a serpentinite melange in the Nishisonogi metamorphic rocks. The jadeitite SBC-115076 research buy mineral assemblage is jadeite/omphacite + paragonite + phlogopite + albite + clinozoisite/epidote +/- muscovite +/- analcime. Jadeite core with fine-grained quartz inclusions and the inclusion-free rim is partially replaced by albite. These jadeitites are surrounded by metasomatic zones of albitites and/or a muscovite rock. The mineral assemblage of the albitites is albite + clinozoisite/epidote +/- muscovite +/- omphacite +/- phlogopite +/- amphibole +/- chlorite, and that of the muscovite rock is muscovite + clinozoisite + chlorite. Because these zones have no high-pressure minerals, they represent products in a P-T regime outside the stability field of jadeite + quartz. Isocon analyses between the jadeitites and the metasomatic zones reveal that K2O, H2O, Sr and Ba were added to jadeitite and SiO2, Na2O and VX-680 chemical structure Fe2O3 were removed. REE-rich veinlets emanate from clinozoisite grains, suggesting REE mobility during the fluid-jadeitite interactions. The metasomatic zones developed by interaction

between jadeitites and serpentinite via K-, Sr- and Ba-rich fluid, during exhumation from the stability field of jadeite + quartz through that of albite to that of analcime.”
“Different analytical techniques were used to find the most reliable and economic method for determining the labile fraction of C in biochar. Biochar was produced from pine, poplar and willow (PI, PO and WI, respectively) at two temperatures (400 and 550 degrees C) and characterised using spectroscopic techniques [solid state (13)C nuclear magnetic resonance spectroscopy (NMR)], molecular markers [pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS)], thermogravimetry (TG), elemental composition and wet oxidation (potassium permanganate and potassium dichromate). Short term incubation (110 h) of an A horizon from an Umbrisol amended with the biochar samples at two doses (7.5 and 15 t ha (1)) was also carried out to provide supplementary information on the influence of biochar-soil interaction on CO(2) evolution.

These data are presented and compared with high level ab initio c

These data are presented and compared with high level ab initio calculations (CCSD(T)/cc-pVTZ). The results obtained https://www.selleckchem.com/products/selonsertib-gs-4997.html in this study are compared with those on acetylene and ethene-HCl complexes. We observe a broadening of the lines interpreted as a decrease of the lifetime of the excited vibrational state with the size of the partner of complexation and also a correlation between the vibrational shift of the H-Cl stretching mode and the proton affinity of the acceptor molecule. (C) 2010 American Institute of Physics. [doi:10.1063/1.3397990]“
“A lot of attention is being paid to potential use of corn stover as a feedstock for bioenergy production.

In addition to meeting renewable energy goals, use of corn stover for energy production may provide a new source of income for corn growers. This study estimates the costs of corn stover harvest and supply, and then uses that information to estimate farm production decisions and changes to farm profit at varying corn stover prices. In this study, corn stover is collected in large round bales using a raking, baling, and staging method. Harvest cost includes payments for fuel, labor, equipment ownership and repair, net wrap, and nutrient replacement. Supply costs include storage, loading and unloading, and

transport. The total cost of harvest and supply is estimated between 82.19 $ Mg-1 (dry) and 100.56 $ Mg-1 (dry). Costs phosphatase inhibitor will vary considerably from farm to farm and from year to year depending on weather conditions.\n\nA linear programming model was used to estimate the willingness of corn growers to harvest corn stover at varying stover prices. Corn stover supply, farm profit, and land allocation was analyzed under

multiple scenarios. At a price of 88.19 $ Mg-1, farms in the base case harvested corn stover at a rate of 2.49 Mg ha(-1) using this website a 33% removal rate. At this price, stover provided enough additional profit to entice farmers to shift to more continuous corn production. Future research is needed to determine the overall impacts of a viable stover market on corn and soybean production and price. (C) 2014 Elsevier Ltd. All rights reserved.”
“Background: Acute scrotal edema is an infrequent complication in patients who undergo continuous ambulatory peritoneal dialysis (CAPD), occurring in 2% to 4% of patients. Inguinal hernia is usually the cause, but the diagnosis is sometimes confusing. Imaging modalities such as computed tomographic peritoneography are helpful but can be equivocal. We have used diagnostic laparoscopy in conjunction with open unilateral or bilateral hernia repair for diagnosis and treatment of peritoneal dialysis (PD) patients with acute scrotal edema.\n\nTechnique and Cases: Three patients with acute scrotal edema while receiving CAPD over the span of 7 years had inconclusive results at clinical examination and on diagnostic imaging.

Main conclusion This study showed that VacA-neutralizing anti

\n\nMain conclusion This study showed that VacA-neutralizing antibodies are significantly associated with gastroduodenal diseases, especially GC, and that they might be used as risk markers of GC and DU.”
“In the title compound, [Ni(NO3)(2)(C24H20N2)(2)], the Ni-II atom has a distorted pseudo-octahedral coordination geometry defined by two chelating nitrate groups and two pyridine N atoms of the monodentate N-(triphenylmethyl)pyridin-2-amine ligands. Intramolecular

N-H center dot center dot center dot O hydrogen bonds help to establish the configuration.”
“Aims.\n\nThe aim of this study was to assess health-related quality of life in patients with inflammatory bowel disease in Zhejiang, Mainland China.\n\nBackground.\n\nThe incidence of inflammatory bowel disease in China is believed to be low but has been increasing www.selleckchem.com/products/anlotinib-al3818.html in the past decade. The quality of life of Chinese patients with inflammatory bowel disease is unknown.\n\nDesign.\n\nA cross-sectional study.\n\nMethods.\n\nThe study was conducted in 92 patients with inflammatory bowel disease in Zhejiang, China, 52

with ulcerative colitis and 40 with Crohn’s disease. Health-related quality of life was measured by the Chinese version of the Inflammatory Bowel Disease Questionnaire and Short Form-36, respectively. Disease activity was assessed by the Walmsley and Harvey-Bradshaw simple AZD8186 indices for ulcerative colitis and Crohn’s disease, respectively. Demographic and clinical variables were also recorded. Short Form-36 data from the study sample were compared with a reference population of 1688 Chinese people residing in Hangzhou, Zhejiang, China.\n\nResults.\n\nNo significant health-related quality of life differences were found between patients with ulcerative colitis and Crohn’s disease (p > 0 center dot 05). Pooled data showed that inflammatory bowel disease patients with active disease had significantly lower scores for all eight dimensions of Short Form-36 compared to those in remission (p < 0 center dot 01); those with active disease scored significantly lower than population norms in all dimensions of Short Form-36 except mental health (p < 0 center

dot 05); whereas those in remission scored significantly lower than population norms in role physical (p < 0 center dot 01) and general VX-680 order health dimensions (p < 0 center dot 05). The regression analyses identified only disease activity index and employment status to explain variations in health-related quality of life (p < 0 center dot 01).\n\nConclusions.\n\nInflammatory bowel disease similarly impairs health-related quality of life in patients with both ulcerative colitis and Crohn’s disease.\n\nRelevance to clinical practice.\n\nThe results suggest that any interventions that produce a stable clinical remission, whether medical or surgical, allowing patients to return to their usual work position can decrease the disease impact on their daily lives.

(C) 2011 Elsevier B V All rights reserved “
“The normal end

(C) 2011 Elsevier B.V. All rights reserved.”
“The normal endothelium inhibits platelet and leukocyte adhesion to the vascular surface maintaining a balance of profibrinolytic and prothrombotic activity. Endothelial function is assessed largely as endothelium-dependent vasomotion, partly based on the assumption

that impaired endothelium-dependent vasodilation reflects the alteration Ferroptosis inhibitor of important endothelial functions. Atherosclerotic risk factors, such as hypercholesterolemia, hypertension, diabetes and smoking, are associated with endothelial dysfunction. In the diseased endothelium, the balance between pro- and antithrombotic, pro- and anti-inflammatory, pro- and antiadhesive or pro- and antioxidant effects shifts towards a proinflammatory, prothrombotic, pro-oxidative and proadhesive phenotype of the endothelium. A common mechanism underlying endothelial dysfunction is related Daporinad to the increased vascular production of reactive oxygen species. Recent studies suggest that inflammation per se, and C-reactive protein in particular, may contribute directly to endothelial dysfunction. The loss of endothelial integrity is a hallmark of atherosclerosis and the causal possible link between each individual risk factor, the development of atherosclerosis and the subsequent clinical events, such as myocardial infarction or stroke.”
“A cyanobacterial strain

(Anabaena laxa RPAN8) exhibiting fungicidal activity and beta-1,3 and 1,4 endoglucanase activities was selected for identifying the gene(s) involved. Functional analyses of the genomic library revealed that four clones (8, 64, 116, and 248) of RPAN8 exhibited fungicidal activity and induced structural deformities

in the cell wall of the growing mycelia of Pythium aphanidermatum. Higher expression of fungicidal and beta-1,4 endoglucanase activities, along with low expression of beta-1,3 endoglucanase activity, was recorded in two E. coli clones (8 and 64). Clones 8 and 64 exhibited identical sequences while clones 116 and 248 were also similar. Bioinformatic analyses were undertaken only for the two non-identical clones 8 and 116 which showed open reading frames (ORFs) of 348 (end 1) and 656 amino acid residues (end 2), respectively. The amino acid sequence analyses revealed that the end 1 encoding endoglucanases buy GNS-1480 belonged to peptidase M20 family while end 2 showed significant similarities with several known genes. The putative promoters and ribosomal binding sites were identified and amino acid exchanges were observed in both end 1 and 2. The presence of signal peptides of 24 and 20 amino acid residues respectively revealed the secretory nature of these proteins.”
“Polymers capable of reversible “two-way” shape memory behaviour are of great interest for applications where reversible actuation is demanded, and semicrystalline crosslinked systems have been indicated as an interesting solution towards this end.

hutchinsi and

O maculatus, although the olfactory sensor

hutchinsi and

O. maculatus, although the olfactory sensory surface area is comparable between these two species and O. ornatus. Orectolobus parvimaculatus has a significantly larger olfactory sensory surface area than the other three species, and there is a positive relationship between total body length and olfactory sensory surface area for all four species. Assuming that these morphological measures are true indications of olfactory capability, the olfactory abilities of wobbegongs are as good as, or better than, other benthic elasmobranchs. Interspecific differences in olfactory ability within this group of benthic ambush predators could indicate relative differences in prey detection, intraspecific recognition and mate detection. Copyright (C) 2009 S. Karger AG, Basel”
“When the central nervous system is the primary affected site in an initial attack of Behcet’s disease (BD), the differential diagnosis is particularly Autophagy inhibitor challenging. Because the specificity of immunobiologic therapy is growing, the specific diagnosis may impact the chosen therapy. For instance, anti-tumour

necrosis factor agents are efficacious in BD but may be harmful in multiple sclerosis or systemic lupus erythematosus. We present two cases with similar neurological features but different diagnosis (BD and systemic lupus erythematosus) as a starting point to review diagnostic and therapeutic approaches for neuro-BD and its differential diagnoses.”
“WARP is a recently described member of the von Willebrand factor A domain superfamily of extracellular matrix proteins, and is encoded by the Vwa l gene. We have Previously shown that WARP is a multimeric BV-6 component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan. However, the tissue-specific expression of WARP in non-cartilaginous

tissues and its localization in the extracellular matrix of selleck chemicals llc other perlecan-containing tissues have not been analyzed in detail. To visualize WARP-expressing cells, we generated a reporter gene knock-in mouse by targeted replacement of the Vwa l gene with beta-galactosidase. Analysis of reporter gene expression and WARP protein localization by immunostaining demonstrates that WARP is a component of a limited number of distinct basement membranes. WARP is expressed in the vasculature of neural tissues and in basement membrane structures of the peripheral nervous system. Furthermore, WARP is also expressed in the apical ectodermal ridge of developing limb buds, and in skeletal and cardiac muscle. These findings are the first evidence for WARP expression in non-cartilaginous tissues, and the identification of WARP as a component of a limited range of specialized basement membranes provides further evidence for the heterogeneous composition of basement membranes between different tissues. (c) 2008 Elsevier B.V.

(C) The Minerals, Metals & Materials Society and ASM Internationa

(C) The Minerals, Metals & Materials Society and ASM International 2013″
“Background/Aims: Low heart rate selleck chemical variability (HRV) is a risk factor for adverse outcomes in the general population. We aimed to determine the factors associated with HRV and evaluate the association between low HRV and clinical outcomes in patients with chronic kidney disease (CKD). Methods: A 10-second electrocardiogram was obtained at baseline in the Chronic Renal Insufficiency Cohort (CRIC) Study. HRV was measured by the standard deviation of all R-R intervals (SDNN) and the root mean square of successive differences between R-R intervals

(RMSSD). Results: In 3,245 CRIC participants with available baseline SDNN and RMSSD, lower HRV was associated with older age, lack

of exercise, heart failure, elevated phosphorus and hemoglobin A1c, and low estimated glomerular filtration rate. After a median follow-up of 4.2 years, in fully adjusted models, lower HRV was not associated with renal [SDNN: hazard rate, HR = 0.96 (95% confidence interval, Cl 0.88-1.05); RMSSD: HR = 0.97 (95% Cl 0.88-1.07)] or cardiovascular outcomes [SDNN: HR = 1.02 (95% Cl 0.92-1.13); RMSSD: HR = 1.00 (95% Cl 0.90-1.10)]. There was a nonlinear relationship between RMSSD and all-cause mortality with increased risk with both low and high RMSSD (p = 0.04). Conclusions: In a large cohort of patients with CKD, multiple risk factors for renal and cardiovascular diseases were YM155 associated with lower HRV. Lower HRV was not associated with increased risk for renal or cardiovascular outcomes, but both low and high RMSSD were associated with increased risk for all-cause mortality. In conclusion, HRV measured Selleck C59 wnt by RMSSD may be a novel and independent

risk factor for mortality in CKD patients. (C) 2013 S. Karger AG, Basel”
“Aims: We showed that chronic cholestatic liver injury induced the expression of c-Myc but suppressed that of glutamate-cysteine ligase (GCL, composed of catalytic and modifier subunits GCLC and GCLM, respectively). This was associated with reduced nuclear antioxidant response element (ARE) binding by nuclear factor-erythroid 2 related factor 2 (Nrf2). Here, we examined whether c-Myc is involved in this process. Results: Similar to bile duct ligation (BDL), lithocholic acid (LCA) treatment in vivo induced c-Myc but suppressed GCL subunits expression at day 14. Nrf2 expression and Nrf2 ARE binding fell markedly. However, Nrf2 heterodimerization with MafG was enhanced by LCA, which prompted us to examine whether LCA treatment in vivo altered proteins that bind to ARE using biotinylated ARE in pull-down assay followed by proteomics. LCA treatment enhanced c-Myc but lowered prohibitin 1 (PHB1) binding to ARE. This was a result of c-Myc-mediated induction of microRNA 27a/b (miR27a/b), which target both PHB1 and Nrf2 to reduce their expression.