Prevena was applied intraoperatively and removed 5 to 7 days postoperatively. The non-Prevena group received either a skin adhesive or absorbent dressing. Groin incisions were assessed, and infection was graded based on Szilagyi classifications. Student t-test and two-sample proportion z test were used for statistical analyses. A P value < .05 was considered statistically significant.
Results: Comorbidities and known risk factors for infection were compared; there were no statistically significant differences between the two groups. Prosthetic material was used in 34 (65%) incisions in the Prevena group and 29 (46%) incisions
in the non-Prevena group. Fifty (96%) incisions within the Prevena group and 60 see more (96%) in the non-Prevena group were classified as clean surgical wounds. Wounds were evaluated at 7 days and 30 days postoperatively. Of 63 groin incisions in 49 patients in the non-Prevena group, 19 (30%) incisions had groin wound
infections. Wound infections were classified into Szilagyi grade I (10; 16%), Szilagyi grade II (7; 11%), and Szilagyi grade III (2; 3%). Of 52 groin incisions in 41 patients in the Prevena group, three (6%) incisions had Szilagyi grade I wound infections. No grade II or III infections occurred in this group. Overall incidence of infection between the two groups was statistically significant (P = .0011).
Conclusions: In this clinical study, HSP inhibitor Prevena negative pressure dressing significantly decreased the incidence of groin wound infection in patients after vascular surgery. (J Vasc Surg 2013;57:791-5.)”
“The distribution of drug delivery systems into the body is affected by plasma proteins adsorbed onto their surface. Furthermore, an exact understanding of the structure and morphology of drug carriers is fundamental to understand
their role as gene delivery systems. In this work, the adsorption of human plasma proteins bound to cationic liposomes Selleckchem Bortezomib and to their relative DNA lipoplexes was compared. A shotgun proteomics approach based on HPLC coupled to high resolution MS was used for an efficient identification of proteins adsorbed onto liposome and lipoplex surfaces. The distinct pattern of proteins adsorbed helps to better understand the DNA compaction process. The experimental evidence leads us to hypothesize that polyanionic DNA is associated to the lipoplex surface and can interact with basic plasma proteins. Such a finding is in agreement with recent results showing that lipoplexes are multilamellar DNA/lipid domains partially decorated with DNA at their surface. Proteomics experiments showed that the lipoplex corona is rich of biologically relevant proteins such as fibronectin, histones and complement proteins. Our results provide novel insights to understand how lipoplexes activate the immune system and why they are rapidly cleared from the blood stream.