The use of hue in optical sensor this website devices has been reported previously, especially in investigations of bitonal optical sensors and of thermochromic liquid crystal thermography. Thus, all relevant color information in digital images of bitonal sensors (sensors in which a chromophore changes into another chromophore with a different spectrum in the presence of a given analyte) is contained in the H coordinate [9, 10]. These authors note that the H coordinate is simple to calculate, is easily obtained from commercial imaging devices, and shows little dependence on variations in color

intensity or variations in brightness of illumination. The reflectance spectra of the thermochromic liquid crystals used in thermography are similar to those of rugate porous silicon, having narrow reflectance peaks with width 30 to 40 nm [11, 12]. These reflectance peaks can move over 100 nm to the blue as temperature www.selleckchem.com/products/bi-d1870.html increases. Thermochromic liquid crystal thermography often relies on a monotonic relationship between hue and temperature. However, several authors have noted that the measured hue is dependent on the illuminant used and is also impacted by background reflectance [11–13]. This can result, PF-02341066 concentration for example, in hue not being monotonic if a red-rich light such as a tungsten lamp is used. Anderson and Baughn noted

that approaches such as subtracting the amount of light in each of the red, green, and blue channels observed at low temperature from all subsequent measurements and then calculating hue using these corrected values could give a monotonic H function for all the light sources they used [11, 12]. They noted that a

monotonic H function was also obtained if they adjusted the white balance of their measurements using the image data corresponding to the low-temperature liquid crystal rather than images of a ‘true gray’ [11]. The concept of deriving a hue-based function after modification of the raw intensity Resveratrol data has been extended further. Thus, Finlayson and Schaefer applied logarithmic preprocessing to obtain a hue parameter that was invariant to brightness and gamma [14], while van der Laak et al calculated absorbance for transmitted light microscopy images prior to determining a hue parameter [15]. There are additional complexities with analyzing digital images of rugate porous silicon compared to thermochromic liquid crystals because the reflectance peaks can be narrower (10 to 30 nm) and the reflectance peak intensities can change to a larger extent with wavelength, due to factors such as light absorption within the porous silicon layer or degradation of the porous layer. In this work, we aimed to use a consumer-grade digital camera to monitor the degradation of freshly etched and modified pSi photonic crystals (rugate filters) rather than using a spectrophotometer.