“Please cite this paper as: Nunes, Krishnan, Gerard, Dale, Maddie, Benton and Hoying (2010). Angiogenic Potential of Microvessel Fragments is Independent of the Tissue of Origin and
can be Influenced by the Cellular Composition of the Implants. Microcirculation17(7), 557–567. We have demonstrated that MFs isolated from adipose retain angiogenic potential in vitro and form a mature, perfused network when implanted. However, adipose-derived Panobinostat microvessels are rich in provascularizing cells that could uniquely drive neovascularization in adipose-derived MFs implants. Objective: Investigate the ability of MFs from a different vascular bed to recapitulate adipose-derived microvessel angiogenesis and network formation and analyze adipose-derived vessel plasticity by assessing whether vessel function ICG-001 cell line could be modulated by astrocyte-like cells. Methods: MFs were isolated by limited collagenase digestion from rodent brain or adipose and assembled into 3D collagen gels in the presence or absence of GRPs. The resulting
neovasculatures that formed following implantation were assessed by measuring 3D vascularity and vessel permeability to small and large molecular tracers. Results: Similar to adipose-derived MFs, brain-derived MFs can sprout and form a perfused neovascular network when implanted. Furthermore, when co-implanted in the constructs, GRPs caused adipose-derived vessels to express the brain endothelial marker glucose transporter-1 and to significantly reduce microvessel permeability. Conclusion: Neovascularization involving isolated microvessel elements is independent of the tissue origin and degree of vessel specialization. In addition, adipose-derived vessels have the ability to respond to environmental signals and change vessel characteristics. “
“Please cite this paper as: Roustit and Cracowski (2012). Non-invasive Assessment of Skin Microvascular Function in Humans:
An Insight Into Methods. Microcirculation 19(1), 47–64. For more than two decades, learn more methods for the non-invasive exploration of cutaneous microcirculation have been mainly based on optical microscopy and laser Doppler techniques. In this review, we discuss the advantages and drawbacks of these techniques. Although optical microscopy-derived techniques, such as nailfold videocapillaroscopy, have found clinical applications, they mainly provide morphological information about the microvessels. Laser Doppler techniques coupled with reactivity tests are widespread in the field of microvascular function research, but many technical issues need to be taken into account when performing these tests. Post-occlusive reactive hyperemia and local thermal hyperemia have been shown to be reliable tests, although their underlying mechanisms are not yet fully understood.