was reviewed for 1272 patients undergoing liver transplantation CHIR 99021 between 1988 and 2012. Clinical outcome was reviewed and their AST level on the third post-operative day was documented. Overall graft survival was calculated on death from any cause or re-transplantation within 3 months, 1, 5 and 10 years. Liver specific death and failure (acute and chronic rejection/primary graft non-function/non-thrombotic infarction/biliary complications) was calculated at 10 years. The AST levels were log transformed and results were analysed via a Full Cox proportional hazard model and T-test. Results were corrected for age, cold ischaemic time and pre-operative creatinine (log transformed). Results 1272 patients 640M/628F/4 Unspecified) were identified. 514 records were excluded from the cox proportional hazard model due to missing creatinine level at day 30. The mean age at time of transplantation was 47 years (37-69). The mean AST level on day 3 post operatively was 613IU/L (Range 18-18885). At all time
points the mean AST levels on day 3 were significantly greater in grafts that failed than those that were still functioning (p<0.001) AST levels on the third post-operative day were found to significantly correlate with overall graft survival (p=0.001) and liver specific failure (p<0.001). For every increase in 1 unit of AST at day 3, the risk of liver specific failure increases by 0.875. Conclusions This retrospective review from a large single centre prospective database has shown that levels of AST on the third post-operative day correlate with long-term clinical outcome following liver transplantation SCH727965 ic50 and would be an adequate outcome measure of trials aimed at reducing IR injury or improving organ preservation. Disclosures: check The following people have nothing to disclose: Francis P. Robertson, Paul R. Bessell, Rafael Diaz-Nieto, Nancy Rolando, Brian R. Davidson Aims: To assess factors associated with cholestasis at 3-months post-liver transplant (LT) and the impact of cholestasis on survival
along with other covariates. Methods: Retrospective cohort study of all (n=489) adult patients who underwent LT at the University of Alberta between 01/2002-12/2012. Cholestasis was defined as either an alkaline phosphatase (ALP) level >2 times the upper limit of normal or a combined elevation of both bilirubin and ALP. Logistic regression was performed to determine independent associations with cholestasis at 3-months post-LT and Cox survival analysis for independent associations with overall survival. Results: 115 patients (24%) had cholestasis at 3-months post-LT. Cholestatic patients were older (54 vs. 52 years, p=0.004) and more likely to be significantly encephalopathic (70% vs. 58%, p=0.017) at the time of LT. Using multivariable logistic regression, independent factors associated with cholestasis at 3-months post LT were age (odds ratio ∼ OR 1.03(1.