Role of Indian academia in preparing the IP ambience
has been highlighted. IEs have been advised to adopt correct IP practices. The preparedness of IEs in the IP matters has been analysed and the gap areas have been identified. Need for a fully functional IP Cell at IE has been established. Such an IP Cell will provide the required support to the inventors and help IE handle its IP obligations. Technology Transfer Office can be a possibility after successful operation of IP Cell at IE.”
“The use of blue native polyacrylamide gel electrophoresis (BN-PAGE) has been reported in the literature to retain both water-soluble and membrane protein complexes in their native hetero-oligomeric state and to determine the molecular Galardin weight of membrane proteins. However, membrane proteins show abnormal mobility when compared with water-soluble markers. Although one could use membrane proteins as markers or apply a conversion factor to the observed molecular weight to account for the bound Coomassie blue dye, when one just wants to assess homo-oligomeric size, these methods appear to be too time-consuming or might not be generally applicable. Here, during detergent screening studies to identify the best detergent for achieving a monodisperse sample, we observed that under certain conditions membrane proteins tend to form ladders of increasing oligomeric
size. Although the ladders themselves Stattic contain no indication of which band represents the correct oligomeric size, they provide a scale selleck kinase inhibitor that can be compared with a single band, representing the native homo-oligomeric size, obtained in other conditions of the screen. We show that this approach works for three membrane proteins: CorA
(42 kDa), aquaporin Z (25 kDa), and small hydrophobic (SH) protein from respiratory syncytial virus (8 kDa). In addition, polydispersity results and identification of the most suitable detergent correlate optimally not only with size exclusion chromatography (SEC) but also with results from sedimentation velocity and equilibrium experiments. Because it involves minute quantities of sample and detergent, this method can be used in high-throughput approaches as a low-cost technique. (C) 2011 Elsevier Inc. All rights reserved.”
“Background: The prognostic value of the New York Heart Association classification (NYHAC) in acutely decompensated heart failure (ADHF) is unknown.\n\nObjectives: We sought to determine the relative value of NYHAC among patients with concomitantly measured amino-terminal pro-B type natriuretic peptide (NT-proBNP) at presentation with ADHF.\n\nMaterials and methods: NYHAC was determined for 720 patients with ADHF and 1-year mortality status was examined. Cox-proportional hazards analysis compared the prognostic accuracy of NYHAC with other ADHF risk measures.\n\nResults: NYHAC had a significant univariate association with 1-year mortality status (HR 1.