Results: The biological properties of Tyr-GX1 peptide were confirmed to be conformable with GX1 in vitro and in vivo. Paper chromatography showed that the labeling efficiency and the radiochemical purity of 131I-Tyr-GX1 peptide were above 90%. The stability of 131I-Tyr-GX1 in different solutions (human serum, mouse serum, PBS, EDTA solution) were maintained about 90 % in 24 h, illustrating that 131I-Tyr-GX1 was stable in vivo or in vitro. Then 24 h SPECT imaging showed increased 131I- labeled GX1 peptide was uptaked by the tumor from 4 h to 24 h gradually after injection, especially in 12–18 h. In biodistribution, high radioactivity was found in kidney, then in the liver, tumor, and
lower radioactivity were found in muscle, brain and bone. The Cerenkov optical signals were collected in abdomen and tumor tissue of nude mice bearing tumor which was identical with the SPECT results. 131I-Tyr-GX1 could cause radiation damage effect to Co-HUVEC and it https://www.selleckchem.com/products/ensartinib-x-396.html had a dependence relationship with concentration. Conclusion: We
obtained a radioactive probe with high labeling efficiency, radiochemical purity and stability, SPECT and Cerenkov optical imagings showed that 131I-Tyr-GX1 have a good tumor-targeting efficacy in vivo. Besides it had a radiation damage effect in vitro and provided references for future internal researches, indicating it may be developed for a new promising gastrointestinal tumor targeted radiotracer. Key Word(s): 1. Tyr-GX1; 2. 131I INCB024360 labeling; 3. Tumor imaging; 4. Radiotherapy; Presenting Author: MEIXIA WANG PJ34 HCl Additional Authors: ZHIJUAN YANG, LIAOLIAO XIN, LI HE, FENXIA LIU, MEIXIU LIU Corresponding Author: MEIXIA WANG Affiliations: Xijing hospital of digestive disease Objective: To compare the efficacy of three-dimensional valve PICC insertion using ultrasonography with the conventional landmark method. Methods: A prospective, randomized study was performed to evaluate the efficacy of three-dimensional valve PICC insertion using ultrasonography with the conventional landmark method
in inpatients planned to receive the chemotherapy in our department. Totally 131 patients were enrolled. 81 cases were assigned to the ultrasound-guided PICC group and other 50 cases were subjected to PICC insertion using the conventional landmark method. The success rate of insertion, as well as relevant complications were compared between two groups including the hyperemia, phlebitis, the catheter-associated bloodstream infection, thrombosis, catheter shifting, number of needle penetrations, time of cannulation and relevant nursing time. Results: Insertion was successful in the 98% of the cases in ultrasound-guided group whereas in 90% of the cases using the conventional landmark method. Patients with ultrasound-guided PICC insertion had fewer complications and improved quality of life compared with the patients with the conventional landmark method.