PubMedCrossRef 27. Marraffini LA: Impact of CRISPR immunity on the emergence of bacterial pathogens. Future Microbiol 2012, 5:693–695.CrossRef 28. Karginov FV, Hannon GJ: The CRISPR system: small RNA-guided defence in bacteria and archaea. Mol Cell 2010, 37:7–19.PubMedCrossRef 29. Rezzonico F, Smits TH, Duffy B: Diversity, evolution, and functionality of clustered regularly interspaced short palindromic repeat (CRISPR) selleck regions in the fire blight pathogen Erwinia amylovora. Appl Environ Microbiol 2011, 77:3819–3829.PubMedCrossRef 30. Barrangou R, Horvath P: CRISPR: new horizons in phage resistance and strain identification. Annu Rev Food Sci Technol 2012, 3:143–162.PubMedCrossRef 31. Brüggemann H, Lomholt HB, Tettelin H,
Kilian M: CRISPR/cas loci of type II Propionibacterium acnes confer immunity against acquisition of mobile
elements present in type I P. acnes. PLoS One 2012, 7:e34171.PubMedCrossRef 32. Rho M, Wu YW, Tang H, Doak TG, Ye Y: Diverse CRISPR evolving in human microbiomes. PLoS Genet 2012, 8:e1002441.PubMedCrossRef 33. Katoh K, Asimenos G, Toh H: Multiple alignment of DNA sequences with MAFFT. Methods Mol Biol 2009, 537:39–64.PubMedCrossRef 34. Crooks GE, Hon G, Chandonia JM, Brenner SE: WebLogo: a sequence logo generator. DAPT Genome Res 2004, 14:1188–1190.PubMedCrossRef 35. Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, Moineau S, Mojica FJ, Wolf YI, Yakunin AF, van der Oost J, Koonin EV: Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol 2011, 9:467–477.PubMedCrossRef 36. Hofacker I: Vienna RNA secondary structure server. Nucleic Acids Res 2003, 31:3429–3431.PubMedCrossRef 37. Weinberger AD,
Sun CL, Pluciński MM, Denef VJ, Thomas BC, Horvath P, Barrangou R, Gilmore MS, Getz WM, Banfield JF: Persisting viral sequences shape microbial CRISPR-based immunity. PLoS Comput Biol 2012, 8:e1002475.PubMedCrossRef 38. Horvath P, Romero DA, Coûtè-Monvoisin AC, Richards M, Deveau H, Moineau S, Boyaval P, Fremaux C, Barrangou R: Diversity, activity, and evolution of CRISPR loci in Streptococcus thermophilus. J Bacteriol 2008, 190:1401–1412.PubMedCrossRef 39. Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V: The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res 2011, mafosfamide 39:9275–9282.PubMedCrossRef 40. Semenova E, Jore MM, Datsenko KA, Semenova A, Westra ER, Wanner B, van der Oost J, Brouns SJ, Severinov K: Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc Natl Acad Sci USA 2011, 108:10098–10103.PubMedCrossRef 41. Mojica FJ, Díez-Villaseñor C, García-Martínez J, Almendros C: Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology 2009, 155:733–740.PubMedCrossRef 42. Swarts DC, Mosterd C, van Passel MW, Brouns SJ: CRISPR interference directs strand specific acquisition.