Most of the phage morphogenesis and replication genes are only expressed at low levels, with many genes (54 of 89 genes) not having any detectable expression (Table 3). In many phages, gene expression and lysogenic conversion occur only when the levels of the repressor protein drop below a certain threshold. None of the STI571 other phages identified in this study had proteins with homology to this putative repressor suggesting that their mechanisms of regulation are different. Table 3 RNASeq analysis of gene expression of phage genes in Bp DD503. Gene Annotation
Expression value (RPKM)* phi1026bp03 putative portal protein 3,601 phi1026bp05 putative major capsid protein 4,743 phi1026bp14
putative tail length tape measure protein 1,038 phi1026bp16 hypothetical protein 3,986 phi1026bp27 putative DNA adenine methylase check details 21,563 phi1026bp28 hypothetical protein 199,000 phi1026bp29 PAAR repeat-containing protein 186,000 phi1026bp30 VRR-NUC domain protein 132,500 phi1026bp31 hypothetical protein 77,624 phi1026bp32 hypothetical protein 8,751 phi1026bp33 hypothetical protein 17,084 phi1026bp34 putative site-specific integrase 5,746 phi1026bp36 hypothetical protein 23,220 phi1026bp37 hypothetical protein 80,994 phi1026bp38 hypothetical protein 16,224 phi1026bp44 hypothetical protein 2,494 phi1026bp48 hypothetical protein 2,501 Entospletinib research buy phi1026bp51 hypothetical protein 26,846 phi1026bp59 putative LysR family transcriptional regulator 18,809 phi1026bp60 putative major facilitator family permease 29,669 phi1026bp61 hypothetical protein 33,472 phi1026bp62 hypothetical
protein 46,783 phi1026bp63 hypothetical protein 10,273 phi1026bp64 hypothetical protein 219,500 phi1026bp65 hypothetical protein 220,000 phi1026bp78 hypothetical protein 4,184 phi1026bp79** putative transcriptional regulator 59,976 phi1026bp81 XRE familiy putative transcriptional regulator 53,561 phi1026bp82 addiction module toxin, RelE/StbE family 92,307 *Genes in bold belong to morons. Only genes with 10 or more reads Baricitinib are displayed, genes with fewer than 10 reads are considered non-expressed since they are not above noise level. Expression values are measured as reads per kilobase of coding sequence per million reads (RPKM). Number of reads and expression values are from one Illumina run, but are representative of 3 runs. **Candidate phage repressor. In addition to the highly expressed repressor, several of the morons in ϕ1026b were also expressed, consistent with the notion that morons are differentially regulated from the rest of the prophage genes as proposed by Hendrix et al . The toxin-antidote morons were highly expressed, with the toxin gene (phi1026bp82) 1.5-fold higher than the antidote gene (phi1026bp81; Table 3).