All the sequences of alleles defined here are freely accessible o

All the sequences of alleles defined here are freely accessible on the website of the Campylobacter MLST website (http://​pubmlst.​org/​campylobacter/​)

developed by Keith Jolley and sited at the University of Oxford [47]. We believe that this tool could be useful for basic surveillance of campylobacteriosis in two ways. For long-term surveillance, it could be combined with MLST data for increased discrimination power, and would help in identifying source attribution of ST complexes shared by more than one sample population: ST21, ST45 and Ponatinib chemical structure ST48 complexes for example [48]. For short term surveillance i.e. detection of temporal clusters of human cases, it could provide some indication on the potential infection source involved when combined with porA or flaA typing [8]. Acknowledgements This

work is a part of the HypoCamp project funded by the National Research Fund of Luxembourg (contract number C09/BM/09). We are grateful to Dr. Keith Jolley and Dr. Alison Cody for publishing our gyrA data on the freely accessible website of the Campylobacter Multi Locus Sequence Typing website: http://​pubmlst.​org/​campylobacter/​. We thank Dr. Martine Denis, Dr. Katell selleck chemical Rivoal (ANSES, Ploufragan, France) as well as Dr. Nadine Botteldoorn and Dr. Sarah Denayer (WIV-ISP, Brussels, Belgium) for providing Campylobacter coli strains of porcine origin. We thank Dr. Christophe Olinger for assistance in the construction of the phylogenetic tree and Dr. Monique Perrin for antimicrobial susceptibility data. Delphine Collard and Cécile Walczak are acknowledged for their environmental sampling efforts and experimental assistance. We thank Dr. Nathalie Welschbillig from the National task Exoribonuclease force “National Priority Campylobacter” for her participation

together with the official veterinarians of the State Veterinary Services and veterinarian practitioners in collecting isolates from veterinarian samples. Additional files Additional file 1: GC contents using concatenated nucleotide sequence: 7 housekeeping genes from MLST with gyrA alleles (3805 bp). Results from the 187 genotypes are classified according gyrA peptide groups. Average in GC% for each group are shown. Additional file 2: Neighbour-joining radial distance phylogenetic tree constructed with concatenated nucleotide sequences from STs identified from this study and from Colles et al. [41] on wild and domesticated ducks. Additional file 3: MICs recorded for C. jejuni isolates with Ser22Gly but without the Thr86Ile substitution. Interpretative thresholds for resistance (R): CIP_R >0.5 and NAL_R > 16. References 1.

Comments are closed.