5 mg/mL) for 15 and 120 min and analyzed

5 mg/mL) for 15 and 120 min and analyzed selleck kinase inhibitor according to the final protocol. Isolates positive at any time point were re-incubated together with the inhibitor suitable for the respective time point (i.e. APBA if hydrolysed within 15 min and DPA if hydrolysed within 2 h).

Isolates negative in the assay were incubated overnight, as well as ertapenem only as negative control, and analysed after 24 h. References 1. Cantón R, Akóva M, Carmeli Y, Giske CG, Glupczynski Y, Gniadkowski M, Livermore DM, Miriagou V, Naas T, Rossolini GM, Samuelsen Ø, Seifert H, Woodford N, Nordmann P: European network on carbapenemases, rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe. Clin Microbiol Infect 2012,18(5):413–431.PubMedCrossRef 2. Giske CG, selleck chemicals Gezelius L, Samuelsen O, Warner M, Sundsfjord A, Woodford N: A sensitive and specific phenotypic assay for detection of metallo-beta-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin. Clin Microbiol Infect 2011,17(4):552–556.PubMedCrossRef 3. Nordmann P, Gniadkowski M, Giske CG, Poirel L, Woodford N, Miriagou V: European Network on Carbapenemases, Identification and screening of carbapenemase-producing

Enterobacteriaceae. Clin Microbiol Infect 2012,18(5):432–438.PubMedCrossRef learn more 4. Sparbier K, Schubert S, Weller U, Boogen C, Kostrzewa M: Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against beta-lactam antibiotics. J Clin Microbiol 2012,50(3):927–937.PubMedCentralPubMedCrossRef 5. Hrabák J, Studentová V, Walková R, Zemlicková H, Jakubu V, Chudácková E, Gniadkowski M, Pfeifer Y, Perry JD, Wilkinson K, Bergerová T: Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 carbapenemases by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012,50(7):2441–2443.PubMedCentralPubMedCrossRef

6. Kempf M, Bakour S, Flaudrops C, Berrazeg M, Brunel JM, Drissi M, Mesli E, Touati A, Rolain JM: Rapid detection of carbapenem resistance in Acinetobacter baumannii using matrix-assisted laser desorption ionization-time 3-mercaptopyruvate sulfurtransferase of flight mass spectrometry. PLoS One 2012,7(2):e31676.PubMedCentralPubMedCrossRef 7. Burckhardt I, Zimmermann S: Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours. J Clin Microbiol 2011,49(9):3321.PubMedCentralPubMedCrossRef 8. Álvarez-Buylla A, Picazo JJ, Culebras E: Optimized method for acinetobacter species carbapenemase detection and identification by matrix-assisted laser desorption J. Clin Microbiol 2013,51(5):1589.CrossRef 9.

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