2d, e, g), which H 89 supplier was NSC23766 supplier followed by a decrease (SSF 650/6; Fig. 2d) or return to the initial level (SSF 1250/12 and SSF 1250/6; Fig. 2e, g) by day 7. We note that the picture
in Fig. 2 remained essentially the same when the QA reduction state was estimated by another parameter (1-ql; data not shown), which takes into account the connectivity among PSII complexes for light energy transfer (Kramer et al. 2004). Fig. 2 Reduction state of Q A (1–qP) during light induction. The measurement protocol and the abbreviations of the light regimes are as described in the legend to Fig. 1. Data are means of five plants (±SE) Inverse patterns were found for ETR (Fig. 3), which is a proxy for the rate of electron transport at PSII. In the C 50 plants, ETR nearly reached saturation at around 80 μmol m−2 s−1 during 8-min illumination at 1,000 μmol photons m−2 s−1 (Fig. 3a). All plants that showed enhancement of QA oxidation during the 7-day acclimation (i.e., C 85, C 120, and LSF 650) also had increasing ETR; on day 7 the ETR values at the end of the
illumination were ca. 100 μmol m−2 s−1 in C 85 and LSF 650 and 120 μmol m−2 s−1 in C 120 (Fig. 3b, c and f). Similarly, the increasing 1-qp detected in the SSF plants (Fig. 2d, e, g) was accompanied by decreasing ETR (Fig. 3d, e, g). The ETR values of these plants were the lowest on day 3 (ca. 60 μmol m−2 s−1), but recovered to 90 (SSF 650/6) or 70 μmol m−2 s−1 (SSF 1250/12 and SSF 1250/6) by day 7. It needs to be reminded, however, that the calculation of ETR based on constant light absorption and equal turnover Selleck Tofacitinib of PSII and PSI (see “Materials and methods”) may not be uniformly applicable to plants undergoing acclimation to different light regimes. Fig. 3 Electron transport rate (ETR) during light induction. The values were calculated from the effective PSII efficiency measured under 1,000 μmol photons m−2 s−1 as described in the legend to Fig. 1. Data are means of five
plants (±SE) Carbohydrate accumulation under different sunfleck conditions In order to see whether the observed changes in PSII activity were reflected in the carbohydrate status of these plants, Glutamate dehydrogenase non-structural carbohydrate was analyzed in mature leaves harvested in the evening (after 10 h of illumination by the different light regimes) on day 2 and 5 (Fig. 4). The concentrations of soluble sugars (the sum of glucose, fructose, and sucrose) varied in leaves under the different light regimes (Fig. 4a), yet the differences between C 50 and other treatments were not significant. Higher starch levels were found in C 85 and C 120 on day 2 (Fig. 4b); especially, the leaf starch content in C 120 was more than three times that of C 50. The starch levels then declined in both C 85 and C 120 by day 5 although the plants in C 120 still had twice as much starch as in C 50. None of these changes in starch was accompanied by similar changes in soluble sugar (Fig. 4a).