1 ± 1.4 yrs, 174 ± 8.7 cm, 78.5 ± 12 kg,) participated in this study. All subjects signed informed consent documents and the study was BI 10773 approved by the Baylor University Institutional Review Board for the Protection of Human Subjects prior to any data collection. Subjects were not allowed to participate in this study if they learn more reported any of the following: 1) current or past history of anabolic steroid use; 2) any metabolic disorders or taking any thyroid, hyperlipidmeic, hypoglycemic, anti-hypertensive, or androgenic medications; 3) ingested any
ergogenic levels of creatine, HMB, thermogenics, ribose, pro-hormones (i.e., DHEA, androstendione, etc.) or other purported anabolic or ergogenic nutritional supplements within 2 months prior to beginning the study; 4) not taking any additional nutritional supplement or contraindicated selleck compound prescription medication during the protocol. Experimental design The study was conducted in a cross-over, randomized, double-blinded,
and placebo-controlled manner. Participants expressing interest in the study were interviewed on the phone/or via email to determine whether they appear to qualify to participate in this study. Participants believed to meet eligibility criteria were then invited to attend an entry/familiarization session. Once reporting to the lab, participants completed a medical history questionnaire and underwent a general physical examination to determine whether they met eligibility
criteria. P-type ATPase Once cleared, participants were familiarized to the study protocol via a verbal and written explanation outlining the study design. All eligible participants who agreed to participate in the study read and signed the university-approved informed consent documents. Participants were familiarized with the angled leg press and leg extension machines, the correct technique in performing each of the exercises, and then performed two low-resistance (30% of body mass) practice/warm-up sets of 10 repetitions on each exercise to familiarize them with the protocol and to also insure that they were able to complete the protocol before being formally admitted to the study. Participants then completed an initial strength test to assess their one repetition maximum (1-RM) for each leg on the angled leg press (Nebula Fitness, Inc., Versailles, OH), and leg extension (Body Masters, Inc., Rayne, LA) exercises using standard guidelines routinely employed our laboratory . Following the practice trials, participants were scheduled to return 48 hours later for testing. Participants were asked to not change their dietary habits in any way throughout the study. This was monitored by having each participant document dietary intake for two days before each testing session.