95, coverage no less than 0.9, and reference gene had annotation of putative or hypothetical. To define genes with signal peptide, we use SignalP version 4.1 ( Petersen et al., 2011) to identify genes with signal peptide with default parameters. TMHMM 2.0 ( Krogh et al., 2001) was used to identify buy EPZ015666 genes with transmembrane helices. The draft genome of B. agri 5-2 revealed a genome size of 5,513,716 bp and a G + C content of 54.15% (146 contigs with N50 of 97,214 bp). These contigs contain 5260 coding sequences (CDSs), 91 tRNAs and 6 incomplete rRNA operons (2 small subunit rRNA and 4 large subunit rRNAs). A total of 5067 protein-coding

genes were assigned as putative function or hypothetical proteins. 3624 genes were categorized into COGs functional groups (including putative or hypothetical genes). The properties and the statistics of the genome are summarized in Table 1. Consistent with its metabolic versatility and environmental LDE225 solubility dmso adaptability, B. agri strain 5-2 possesses extensive transport capabilities. 517 genes encode transport related proteins for amino acid, inorganic ions, carbohydrates, nucleotide and lipid found in the genome. Two putative alkane 1-monooxygenase, one putative alkanesulfonate monooxygenase, one putative alkanesulfonate transporter, one putative sulfate permease and five putative sulfate transporters were identified in the draft genome

( Table 2). Alkane 1-monooxygenase was found as one of the key enzymes responsible for the aerobic transformation of midchain-length n-alkanes (C5 to C16) and in some cases even longer

alkanes ( van Beilen and Funhoff, 2007). It is hypothesized that sulfate transporters and alkanesulfonate transporter may be responsible for organosulfur compound degradation ( Erwin et al., 2005 and Van Hamme et al., 2013). Moreover, a genome alignment of the only two sequenced B. agri genomes (B. agri 5-2 and B. agri BAB-2500 ( Joshi et al., 2013)) Akt inhibitor showed that some functional regions are highly homologous between two assemblies. The alignment also reveals some discrepancies between them, some short stretches of the 5-2 genome absent from the contigs in BAB-2500 ( Fig. 1). For example, none of the alkane monooxygenases were identified in the genome of BAB-2500 by further genomic analysis. In summary, the genomic data of strain 5-2 may provide insights into the mechanism of microorganisms adapt to the petroleum reservoir after chemical flooding. This whole genome sequence project is deposited in DDBJ/EMBL/GenBank under the accession JATL00000000. The following are the supplementary data related to this article. Fig. S1.   Phylogenetic tree highlighting the position of Brevibacillus agri strain 5-2 relative to other type strains within the genus Brevibacillus. Numbers at the branching nodes are percentages of bootstrap values based on 1000 replications.