25 mm, film 0.25 mm. The operating conditions were as follows: flow rate = 1.0 mL/min; linear velocity of 24 cm/s; detector temperature of 280 °C; injector temperature of 250 °C; oven Sirolimus temperature of 110°C-5 min/110–215 °C – 5 °C/min/215 °C = 34 min; stripping gas: helium; volume injected 1.0 μL; split 1:50. In order to have a graphical and numerical view of the amount of n-3 EE encapsulated was
determined using the mean results of total lipid content obtained to calculate the encapsulation efficiency (2.2.2), which were multiplied by the EPA + DHA concentration obtained in the fatty acid composition (2.2.6). The evaluation of the effects of different concentrations of wall material (SPI:GA – x1), core material (wall:core – x2) and reticulating agent (TG – x3) on the characteristics of the EE microcapsules, was carried out using the STATISTICA 7.0 (StatSoft, Inc., Tulsa,
OK, USA) software, following a 23 central compound selleck compound rotational design (CCRD), with 6 axial points and 4 central points, verifying the possibility of analyzing the results by response surface methodology, where the results of regression coefficients to encapsulation process yield were determined. The same program and trials were used for the means comparison test (verifying differences between trials 19 and 20) by the analysis of variance (ANOVA) and Tukey’s test, at a significance level of 95% (p ≤ 0.05). Table 1 shows the values obtained for encapsulation process yield and encapsulation efficiency, and Table 2 shows the analysis of variance of the mathematical models obtained for encapsulation process yield. Equation (3) shows the complete regression model (R2 = 0.92; Fcalc/Ftab = 2.98) obtained for the encapsulation process yield (EY). Based on the coefficient of determination (R2), the regression model explained 92% of the responses. equation(3) EY=yi=47.56−3.91×1−1.72×12−2.91×2−1.22×22+0.11×3−0.43×32+1.21x1x2−0.48x1x3−0.68x2x3 Fig. 1 shows the response
surfaces and contour curves obtained for encapsulation process yield, which showed that the effects of the wall material (SPI-GA) concentration and the wall material to core material ratio (wall:core) presented more significant effects than the other variables. Fig. 1 shows that the smaller the core material concentration and the higher the SPI:GA ratio, the higher the encapsulation process yield, the maximum value being obtained for C5 (1.8:1.0 pheromone SPI:GA; 2.6:1.0 wall:core; 8.38 UA de TG/g) approximately 54 g/100 g. These results corroborate those cited in the literature. Jun-xia et al. (2011) found the maximum values for encapsulation yield when they used only 10 g/100 g core material (orange essential oil) in relation to the wall material (SPI:GA), the values falling with increases in core concentration. Lamprecht, Schafer, and Lehr (2001) obtained close to 90 g/100 g encapsulation yield for capsules of fish oil ethyl esters encapsulated in a matrix of gelatin and GA by complex coacervation.